How much glucose is high in DMEM?
Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.
Does DMEM contain glucose?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What is DMEM GlutaMAX?
DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
What is the role of DMEM in cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
How can I get 10 FBS?
(When you make 10% FBS, 1% Pen/Strep-containing DMEM, you just directly add 50 ml of FBS and 5 ml of Pen/Strep.)
Why is DMEM red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).
Does fetal bovine serum contain glucose?
the cells will be suffered high glucose DMEM (25.0 mM glucose) or glucose-free DMEM (no glucose) 24h, low glucose as control(5.55 mM glucose). However, the serum contain glucose.
Is GlutaMAX the same as L-glutamine?
there is difference between applying GlutaMAX and L-Glutamine. Nonetheless they function the same way as a culture media supplement (normally added around 2 mM). GlutaMAX is a derived form of L-glutamine, in the form of L-alanyl-L-glutamine, which prevents the accumulation of ammonia (which is toxic in cell cultures).
Is GlutaMAX glutamine?
Gibco GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture media. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.
What is the disadvantage of serum?
The disadvantages of serum are described, including variability, shelf life, availability, effect on down-stream processing, and potential for contamination.