What does FPIA stand for?
FPIA
| Acronym | Definition |
|---|---|
| FPIA | Fournitures Plastiques Industries Arrosage (French: Watering Supplies Plastics Industries) |
| FPIA | Financial Planners and Insurance Advisors |
| FPIA | Fort Payne Improvement Authority |
| FPIA | Flourescence Polarization Immunoassay |
What is a fluoroimmunoassay?
Fluoroimmunoassay. The use of fluorescence spectrometry to obtain quantitative results for the FLUORESCENT ANTIBODY TECHNIQUE. One advantage over the other methods (e.g., radioimmunoassay) is its extreme sensitivity, with a detection limit on the order of tenths of microgram/liter.
How is fluorescence polarization immunoassay measured?
Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization. The change in polarization is proportional to the amount of antigen in sample, and is measured by a fluorescence polarization analyzer.
What is the principle in fluorescence immunoassay?
A modern fluorescent based immunoassay uses as the detection reagent a fluorescent compound which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.
What is the principle of immunological test?
The immunological tests used in laboratories are made by producing artificial antibodies that exactly “match” the substance or germ in question. When these antibodies come into contact with a sample of blood, urine or stool, they bind to the matching substance or germ if found in the sample.
What is the purpose of immunoassays?
Immunoassays are biochemical tests used to detect the presence or concentration of a specific chemical, such as a toxin or hormone, in a solution using antibody-antigen reactions.
Which is the best description of the FPIA assay?
FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the requirement of separation or washing steps. The basis of the assay is fluorescence anisotropy, also known as fluorescence polarization.
How does FPIA relate to change in fluorescence polarization?
FPIA quantifies the change in fluorescence polarization of reaction mixtures of fluorescent-labelled tracer, sample antigen, and defined antibody. Operating under fixed temperature and viscosity allows for the fluorescence polarization to be directly proportional to the size of the fluorophore.
Which is more accurately measured by FPIA or emit?
Automated analyzers, such as TDx, ADx, and Axysm (Abbott Laboratories), are used to measure these reactions. Drugs are more accurately measured by FPIA than EMIT because sample matrices have less effect on changes in fluorescein polarization. A. Sharma, S.N. Hajare, in Encyclopedia of Food Microbiology (Second Edition), 2014
How does FPIA work between antibody and tracer?
FPIA involves a competitive binding reaction between antibody, drug and a tracer, which is typically fluorescein-labeled drug. The antibody can bind both the drug in the specimen and in the tracer. If the drug concentration in the specimen is high, more of the tracer will remain unbound.