What is p300 in genetics?
Collapse Section. The EP300 gene provides instructions for making a protein called p300, which regulates the activity of many genes in tissues throughout the body. This protein plays an essential role in controlling cell growth and division and prompting cells to mature and take on specialized functions (differentiate) …
What does the p300 protein do?
Function. p300 HAT functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
What is p300 Acetylate?
p300 Acetyltransferase Activity. p300/CBP has a central acetyltransferase (also called HAT) domain that catalyzes the reaction in which the acetyl group from acetyl-CoA is transferred to a protein lysine side chain. p300/CBP has the ability to acetylate a wide variety of proteins (see Supporting Information Table 1).
Is EP300 an oncogene?
EP300 acts as an oncogene affecting ESCC cell growth, colony formation, cell migration and invasion.
Is p300 a protein?
EP300 (E1A Binding Protein P300) is a Protein Coding gene. Diseases associated with EP300 include Rubinstein-Taybi Syndrome 2 and Menke-Hennekam Syndrome 2.
What does CBP protein do?
CREB-binding protein (CBP) and its homologue p300 are transcriptional co-activators of various sequence-specific transcription factors that are involved in a wide array of cellular activities, such as DNA repair, cell growth, differentiation and apoptosis.
Is CBP a transcription factor?
CBP and p300 are transcriptional coactivators that physically interact with diverse sequence-specific DNA-binding factors through conserved domains.
What Acetylates h3k27?
H3K27ac is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates acetylation of the lysine residue at N-terminal position 27 of the histone H3 protein. H3K27ac is associated with the higher activation of transcription and therefore defined as an active enhancer mark.
What does acetylation of histones do?
Acetylation of histones alters accessibility of chromatin and allows DNA binding proteins to interact with exposed sites to activate gene transcription and downstream cellular functions.
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Which is the best anti P300 antibody for humans?
Anti-p300 Antibody (F-4) is a mouse monoclonal IgG 2a κ p300 antibody, cited in 59 publications, provided at 200 µg/ml. raised against amino acids 774-1045 of p300 of human origin. Anti-p300 Antibody (F-4) is recommended for detection of p300 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. Anti-p300 Antibody (F-4) is available
What kind of cancer can be caused by EP300?
Defects in EP300 may play a role in epithelial cancer. Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t (8;22) (p11;q13) with KAT6A.
How is the P300 responsive gene de-repressed?
The CRD1 domain (cell cycle regulatory domain 1) mediates transcriptional repression of a subset of p300 responsive genes; it can be de-repressed by CDKN1A/p21WAF1 at least at some promoters. It conatins sumoylation and acetylation sites and the same lysine residues may be targeted for the respective modifications.