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## What is the persistence length of single stranded DNA?

As is known, the persistence length and the length per base of dsDNA is 50 nm [46] and 0.34 nm [47] respectively. The persistence length of single-stranded DNA, as a kind of flexible polymer, cannot be larger than 50 nm.

## How would increasing the salt concentration change the persistence length of dsDNA?

As expected, with increasing salt concentration a pronounced decrease is observed in the persistence length. The measured persistence length varied from about 25 nm at high ionic force (100 mM NaCl) to about 85 nm at lower ionic force (1 mM NaCl).

Why persistence length is important?

The persistence length is a useful measure of the degree of structural rigidity of a polymer chain and the energy cost of deforming it 1., 2., 3., 4..

What is the length of double stranded DNA?

In an aqueous solution, the average persistence length is 46–50 nm or 140–150 base pairs (the diameter of DNA is 2 nm), although can vary significantly. This makes DNA a moderately stiff molecule.

### How do I calculate ssDNA length?

Estimating the contour length of ssDNA Before making comparison to theoretical models, we need to estimate the unit length of each base b0 so that the entire length of a fully stretched ssDNA of N bases, or the contour length L, is given by L = b0N.

### What does a high persistence length mean?

Such large persistent length for spaghetti does not mean that it is not flexible. It just means that its stiffness is such that it needs. m of length for thermal fluctuations at 300K to bend it. Another example: Imagine a long cord that is slightly flexible.

How is Kuhn length calculated?

For worm-like chain, Kuhn length equals two times the persistence length.

How do you calculate persistence length?

with a persistence length P = E/kBT. C(s) = <ˆu(s) · ˆu(0)>. Hence, a large persistence length means directional accordance of averaged tangent chain vectors over large distances on the chain’s contour. A large persistence length is therefore an in- dication for stiffness.

## Why is double stranded DNA more stable?

The double-stranded helical structure of DNA is maintained primarily by the hydrogen bonds, which are weak bonds. Therefore, double-stranded DNA with a higher number of G-C base pairs will be more strongly bonded together, more stable, and will have a higher melting temperature.

## Can viruses have double stranded DNA?

Any of the viruses belonging to the Class I of Baltimore classification system characterized by having a double stranded DNA as the genetic material and using a DNA-dependent DNA polymerase during replication. Most of these viruses must enter the host nucleus before they can replicate.

What is contour length of DNA?

The contour length is equal to the number of monomers in the chain times the distance per monomer (0.338 nm/bp for B-form DNA). The persistence length characterizes the bending stiffness of the polymer and depends on the molecule’s local elastic behavior.

Is RNA or DNA double stranded?

DNA double helix means that the two-stranded structure of DNA structure is common knowledge, RNA’s single stranded format is not as well known. RNA can form into double-stranded structures, such as during translation, when mRNA and tRNA molecules pair.

### Why is the persistence length of DNA increased?

The value 55 nm of the persistence length of DNA is much larger than the 7 nm it would be if the phosphate charge were lacking. The enhancement of persistence length is due to the electrostatic tension within the DNA double helix generated by phosphate-phosphate repulsions.

### What is the persistence length of DNA in NaCl?

DNA is among the stiffest of known polymers with a persistence length of ∼50 nm (150 bp) in 0.1 M aqueous NaCl. Despite that, we have calculated a mere 6% neutralization of phosphate groups as enough to reduce to zero the energy penalty of bending DNA to its nucleosomal radius of curvature (16).

How is the persistence length of a chain calculated?

The persistence length equals the average projection of the end-to-end vector on the tangent to the chain contour at a chain end in the limit of infinite chain length. The persistence length can be also expressed using the bending stiffness , the Young’s modulus E and knowing the section of the polymer chain.

What is the persistence length of a tangent?

Formally, the persistence length, P, is defined as the length over which correlations in the direction of the tangent are lost.