How does BCA assay calculate protein concentration?
You can use this formula to calculate the amount of protein in your sample: [ (OD of the test sample) / (OD of the standard sample) ] X concentration of the standard sample. = 4.0 mg/mL protein (in test sample).
How does Bradford assay calculate protein concentration?
To calculate the concentration of the undiluted, unknown sample, simply multiply by the dilution factor. So, 0.5 x 10= 5mg/ml.
Is BCA assay accurate?
BCA is sensitive and has a broad dynamic range; capable of measuring protein concentrations of 0.5 μg/mL to 1.5 mg/mL. Substances that reduce copper will also produce colour in the BCA assay, thus interfering with the accuracy of the protein quantitation.
How does the Pierce BCA protein assay work?
The Pierce BCA Protein Assays combines the well-known reduction of Cu2+ to Cu+ by protein in an alkaline medium (also known as the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+) by bicinchoninic acid (BCA). Figure 1.
What is the purpose of the BCA assay?
The BCA Protein Assay is a popular method for colorimetric detection and quantitation of total protein.
What is the protocol for Thermo Scientific Pierce Protein Assay?
The Micro BCA Protein Assay Standard Protocol: 60°C for 60 minutes 0.5-20µg/mL (0.5mL) Microplate Protocol: 37°C for 120 minutes 2-40µg/mL (150µL)
What are the advantages of Thermo Scientific Protein Assay?
Thermo Scientific Compat-Able Protein Assays 26 Modified Lowry Protein Assay 27 Chemistry of the Modified Lowry Protein Assay 27 Advantages of the Modified Lowry Protein Assay 28 Disadvantages of the Modified Lowry Protein Assay 28 General Characteristics of the Modified Lowry Protein Assay 28 Modified Lowry Protein Assay Reagent 29