What is MTS in MTS assay?
The MTS assay is designed to evaluate the metabolic activity and viability of cells. The assay involves the conversion of the tetrazolium salt MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to a purple formazan in the presence of phenazine methosulfate.
What is MTS cell proliferation assay?
The MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media.
What is the difference between MTT and MTS assay?
The main difference between MTT and MTS assay is that MTT assay has an additional step associated with the solubilization of formazan crystals whereas MTS assay is not associated with the solubilization of formazan crystals. MTT and MTS assay are two types of assays used to measure cell viability in vitro.
How is MTS assay calculated?
- Subtract the absorbance of the blank wells from all the wells.
- Divide the absorbance of the wells which have the cells treated with the drug/inhibitor by the average of the absorbances emitted from the cells in the control wells.
- Multiply the ratio by 100 to give you the viability in %.
Is MTS toxic to cells?
The MTS is an alternative to MTT and the formazan formed from MTS is water-soluble and less toxic . Theoretically, the color intensity of the formazan dye is correlated to the number of viable cells.
How many cells are needed for MTS assay?
A titration of cell numbers between 1,000 & 10,000 per well would be a good place to start. its depend on cell type and its growth rate; and also on number of days of your experiment.
What is the purpose of MTS assay?
The MTS assay is used to assess cell proliferation, cell viability and cytotoxicity. The MTS assay protocol is based on the reduction of the MTS tetrazolium compound by viable mammalian cells (and cells from other species) to generate a colored formazan dye that is soluble in cell culture media.
How does the MTS assay work?
The MTS assay protocol is based on the reduction of the MTS tetrazolium compound by viable mammalian cells (and cells from other species) to generate a colored formazan dye that is soluble in cell culture media. The formazan dye is quantified by measuring the absorbance at 490-500 nm.
How long is MTS assay?
1 to 4 hours
MTT Tetrazolium Assay Concept The MTT substrate is prepared in a physiologically balanced solution, added to cells in culture, usually at a final concentration of 0.2 – 0.5mg/ml, and incubated for 1 to 4 hours.
Can cell viability be more than 100?
Yes indeed, you can get more than 100%. Yes, In case of cellular proliferation we can get higher percentage of cellular viability using calculation of ratio between treated cell and non-treated/standard cell OD values in percentage.
How do you calculate viability?
To calculate viability:
- Add together the live and dead cell count to obtain a total cell count.
- Divide the live cell count by the total cell count to calculate the percentage viability.
What is a good cell viability?
A good cell viability is anywhere between 80-90% in most of the cell lines.
Is there an alternative to the MTT assay kit?
An alternative product, MTS assay kit ab197010, uses a similar principle to this kit, but without the need for the MTT solvent step. Store at -20°C. Please refer to protocols. Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
When to use the XTT assay kit ab232856?
XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. This provides insights into cell viability, cell proliferation, and cytotoxicity.
How is the MTS assay performed in cell culture?
The MTS assay protocol is performed by adding the MTS reagent directly into the cell culture media, without the intermittent steps which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in a 96-well microtiter plate.
Is the MTT assay based on a water soluble product?
The MTT assay protocol is based on the conversion of water soluble MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan. Dead cells lose this ability and therefore show no signal.